钙非依赖性磷脂酶A2(iPLA2)重组蛋白

钙非依赖性磷脂酶A2(iPLA2)重组蛋白

Recombinant Phospholipase A2, Calcium Independent (iPLA2)

PLA2G6; CaI-PLA2; GVI; INAD1; PNPLA9; Ca2+ Independent PLA2; 85 kDa Calcium-Independent Phospholipase A2; Phospholipase A2,group VI(Cytosolic,Calcium-Independent)

[ PROPERTIES ]

Residues: Leu481~Val702 (Accession # P97819),

with two N-terminal Tags, His-tag and T7-tag. Host: E. coli

Subcellular Location: Membrane; Peripheral

membrane protein. Cytoplasm.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 9.5

Predicted Molecular Mass: 28.3kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LLCLDGGGVK GLVIIQLLIAIEKASGVATK DLFDWVAGTS TGGILALAIL HSKSMAYMRG VYFRMKDEVF RGSRPYESGPLEEFLKREFG EHTKMTDVKK PKVMLTGTLS DRQPAELHLF RNYDAPEAVR EPRCNQNINLKPPTQPADQL VWRAARSSGA APTYFRPNGR FLDGGLLANN PTLDAMTEIH EYNQDMIRKGQGNKVKKLSI VVSLGTGKSP QV

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