抑制素βB(INHbB)重组蛋白

抑制素βB(INHbB)重组蛋白

Recombinant Inhibin Beta B (INHbB)

INH-BB; Activin AB Beta Polypeptide

[ DESCRIPTION ]

Protein Names: Inhibin Beta B

Synonyms: INHbB

Species: Mouse

Size: 100µg

Source: Escherichia coli-derived

Subcellular Location: Secreted.

[ PROPERTIES ]

Residues: Gly297~Ala411 (Accession # Q04999),

with N-terminal His-Tag.

Grade & Purity: >95%, 16kDa as determined by

SDS-PAGE reducing conditions.

Formulation: Supplied as lyophilized form in PBS, pH

7.4, containing 5% sucrose.

Endotoxin Level: <1.0 EU per 1µg (determined by

the LAL method).

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Molecular Mass: 14.3kDa

Predicted isoelectric point: 5.9

Mouse INHbB

[ PREPARATION ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

 Store at 2-8oC for one month.

 Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSGSEF-GLEC DGRTSLCCRQ QFFIDFRLIG WNDWIIAPTG YYGNYCEGSCPAYLAGVPGS ASSFHTAVVN QYRMRGLNPG PVNSCCIPTK LSSMSMLYFD DEYNIVKRDVPNMIVEECGC A

[ REFERENCES ]

1. Hu B., et al. (2012) Cell 149:1207-1220.

2. Koss M., et al. (2012) Dev. Cell 22:913-926.

3. Gokoffski K.K., et al. (2011) Development 138:4131-4142.

4. Gokoffski K.K., et al. (2011) Development 138:4131-4142. 

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