碳酸酐酶Ⅳ(CA4)重组蛋白

碳酸酐酶Ⅳ(CA4)重组蛋白

Recombinant Carbonic Anhydrase IV (CA4)

CA-IV; CAIV; Car4; RP17; Retinitis Pigmentosa 17(Autosomal Dominant); Carbonate dehydratase IV

[ DESCRIPTION ]

Protein Names: Carbonic Anhydrase IV

Synonyms: CA4, Car4

Species: Mouse

Size: 100µg

Source: Escherichia coli-derived

Subcellular Location: Cell membrane; Lipid-anchor,

GPI-anchor.

[ PROPERTIES ]

Residues: Gly21~Ser216 (Accession # Q64444), with

N-terminal His-Tag.

Grade & Purity: >95%, 25kDa as determined by

SDS-PAGE reducing conditions.

Formulation: Supplied as lyophilized form in PBS, pH

7.4, containing 5% sucrose, 0.01% sarcosyl.

Endotoxin Level: <1.0 EU per 1µg (determined by

the LAL method).

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Molecular Mass: 23.5kDa

Predicted isoelectric point: 7.8

Mouse CA4

[ PREPARATION ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

 Store at 2-8oC for one month.

 Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSGSEF- GWCYEIQTKD PRSSCLGPEK WPGACKENQQ SPINIVTARTKVNPRLTPFI LVGYDQKQQW PIKNNQHTVE MTLGGGACII GGDLPARYEA VQLHLHWSNGNDNGSEHSID GRHFAMEMHI VHKKLTSSKE DSKDKFAVLA FMIEVGDKVN KGFQPLVEALPSISKPHSTS TVRESSLQDM LPPSTKMYTY FRYNGS 

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