通用转录因子ⅡH肽1(GTF2H1)重组蛋白

通用转录因子ⅡH肽1(GTF2H1)重组蛋白

Recombinant General Transcription Factor IIH Subunit 1 (GTF2H1)

GTF2-H1; BTF2; TFIIH; BTF2 p62; Basic transcription factor 2 62 kDa subunit; TFIIH basal transcription factor complex p62 subunit; General Transcription Factor IIH, Polypeptide 1

[ PROPERTIES ]

Residues: Thr84~Met335 (Accession # P32780),

with two N-terminal Tags, His-tag and T7-tag. Host: E. coli

Subcellular Location: Nucleus.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 8.4

Predicted Molecular Mass: 32.0kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS - TAVKERD AVKDLLQQLLPKFKRKANKE LEEKNRMLQE DPVLFQLYKD LVVSQVISAE EFWANRLNVN ATDSSSTSNHKQDVGISAAF LADVRPQTDG CNGLRYNLTS DIIESIFRTY PAVKMKYAEN VPHNMTEKEFWTRFFQSHYF HRDRLNTGSK DLFAECAKID EKGLKTMVSL GVKNPLLDLT ALEDKPLDEGYGISSVPSAS NSKSIKENSN AAIIKRFNHH SAMVLAAGLR KQEAQNEQTS EPSNM

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！