蛋白二硫化物异构酶A6(PDIA6)重组蛋白

蛋白二硫化物异构酶A6(PDIA6)重组蛋白

Recombinant Protein Disulfide Isomerase A6 (PDIA6)

PDI-A6; TXNDC7; P5; ERp5; Thioredoxin Domain Containing 7(Protein Disulfide Isomerase); Protein Disulfide Isomerase-Associated 6; Endoplasmic reticulum protein 5

[ PROPERTIES ]

Residues: Leu20~Leu440

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q15084

Host: E. coli

Subcellular Location: Endoplasmic reticulum lumen.

Cell membrane. Melanosome.

Purity: >90%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 5.0

Predicted Molecular Mass: 49.9kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

L YSSSDDVIEL TPSNFNREVI QSDSLWLVEF YAPWCGHCQR LTPEWKKAAT ALKDVVKVGAVDADKHHSLG GQYGVQGFPT IKIFGSNKNR PEDYQGGRTG EAIVDAALSA LRQLVKDRLGGRSG GYS SGK QG RS DSS SK K DVIE LT D DS F DK NV L DS E DV WMVE FYAPW CGHCKNLEPEW AAAASEVKEQ TKGKVKLAAV DATVNQVLAS RYGIRGFPTI KIFQKGESPVDYDGGRTRSD IVSRALDLFS DNAPPPELLE IINEDIAKRT CEEHQLCVVA VLPHILDTGAAGRNSYLEVL LKLADKYKKK MWGWLWTEAG AQSELETALG IGGFGYPAMA AINARKMKFALLKGSFSEQG INEFLRELSF GRGSTAPVGG GAFPTIVERE PWDGRDGELP VEDDIDLSDVELDDLGKDEL

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