含亮氨酸丰富重复蛋白3C(LRRC3C)重组蛋白

含亮氨酸丰富重复蛋白3C(LRRC3C)重组蛋白

Recombinant Leucine Rich Repeat Containing Protein 3C (LRRC3C)

[ PROPERTIES ]

Residues: Val42~Val275

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: A6NJW4

Host: E. coli

Subcellular Location: Membrane; Single-pass

membrane protein.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 5.9

Predicted Molecular Mass: 28.7kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

VPSPQVPPR GCYVAKEAGE RTFRCSQAGL SAVPSGIPND TRKLYLDANQ LASVPAGAFQHLPVLEELDL SHNALAHLSG AAFQGLEGTL RHLDLSANQL ASVPVEAFVG LQIQVNLSANPW H C D CA L Q E V L R Q V R LV P G TG T G I V C G S G A R P D LV G Q E F L L L A G E E E L CGSGWGGARRS TDVALLVTMG GWLTLMVAYL VHYVWQNRDE TRRSLKRAPV LPVRSEDSSILSTVV

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