粘附调节分子1(ADRM1)重组蛋白

粘附调节分子1(ADRM1)重组蛋白

Recombinant Adhesion Regulating Molecule 1 (ADRM1)

GP110; Rpn13; Proteasomal Ubiquitin Receptor ADRM1; 110 kDa cell membrane glycoprotein; Proteasome regulatory particle non-ATPase 13

[ PROPERTIES ]

Residues: Thr2~Asp407

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q16186

Host: E. coli

Subcellular Location: Cytoplasm. Nucleus.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 5.6

Predicted Molecular Mass: 45.8kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

TTSGALFPS LVPGSRGASN KYLVEFRAGK MSLKGTTVTP DKRKGLVYIQ QTDDSLIHFCWKDRTSGNVE DDLIIFPDDC EFKRVPQCPS GRVYVLKFKA GSKRLFFWMQ EPKTDQDEEHCRKV NEY L NN PPMPGA LGAS GSSGHE LSA L GGEG G LQS L L GNMS HSQ LMQLIGPAGLGGL GGLGALTGPG LASLLGSSGP PGSSSSSSSR SQSAAVTPSS TTSSTRATPAPSAPAAASAT SPSPAPSSGN GASTAASPTQ PIQLSDLQSI LATMNVPAGP AGGQQVDLASVLTPEIMAPI LANADVQERL LPYLPSGESL PQTADEIQNT LTSPQFQQAL GMFSAALASGQLGPLMCQFG LPAEAVEAAN KGDVEAFAKA MQNNAKPEQK EGDTKDKKDE EEDMSLD

[ REFERENCES ]

1. Shimada S., et al. (1994) Cancer Res. 54:3831-3836. 

2. Joergensen J.P., et al. (2006) J. Mol. Biol. 360:1043-1052. 

3. Yao T., et al. (2006) Nat. Cell Biol. 8:994-1002. 

4. Fejzo M.S., et al. (2008) Genes Chromosomes Cancer 47:873-883.

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！