干扰素α(IFNa)重组蛋白

干扰素α(IFNa)重组蛋白

Recombinant Interferon Alpha (IFNa)

IFNA1; IFL; LeIF D; IFN; IFN-Alpha; IFNA13; IFN-A; IFNAP22; IFN-Alpha 1b; Interferon Alpha 1b; Interferon, Leukocytic; IFN, Leukocyte; Interferon alpha-D

[ PROPERTIES ]

Residues: Ser1~Val93

Tags: Two N-terminal Tags, His-tag and GST-tag

Accession: Q75T05

Host: E. coli

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 0.05% sarcosyl.

Predicted isoelectric point: 4.4

Predicted Molecular Mass: 40.3kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

SCLKDRKDFG FPLEKVDAQQ IQKAQAISIL HELSQQVLNI YTSXDSSAAW DATLLDSFCNDLQQQLSGLQ ACQMHQVGVQ EPPLAQEDSL LAV

[ REFERENCES ]

1. Weerd NA., et al. (2007) J Biol Chem 282 (28): 20053–20057.

2. Navratil V., et al. (2010) Journal of Proteome Research 9 (7): 3527–36.

3. Paolicelli., et al. (2009) Biologics: Targets & Therapy 3: 369–376.

4. Ge D., et al. (2009) Nature 461 (7262): 399–401.

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