丝氨酸棕榈酰转移酶长链碱性亚基3(SPTLC3)重组蛋白

丝氨酸棕榈酰转移酶长链碱性亚基3(SPTLC3)重组蛋白

Recombinant Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3)

SPTLC2L; SPT 3; LCB 3; C20orf38; Aminotransferase 2; Serine-palmitoyl-CoA transferase 3; Long chain base biosynthesis protein 3

[ PROPERTIES ]

Residues: Arg80~Ala563

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q8BG54

Host: E. coli

Subcellular Location: Endoplasmic reticulum

membrane. Single-pass membrane protein.

Purity: >90%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 7.3

Predicted Molecular Mass: 58.4kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

R DFMRNWGIEK CNAAVEREEQ KDFVPLYQDF ENFYKRNLYM RI RDSWSHTVCSAPEPYMNV MEKVTDDYNW TFRHTGKVIE NIINMASYNY LGLAGKYDDS MVRVKDTLEKYGVGVASTRN EMGTLDIHKE LEDLMAEFLN VEAVMSFGMG FATNAMNIPV FVGKGCLILSDEFNHTSVIL GSRLSGAVIR PFKHNNAENL EKLLREAIIR GQPGTGRAWK KILIVVEGVYSMEGSIVNLA QIVALKKKYK AYLYIDEAHS IGCTGPTGRG VRELFGLDPE DIDVYMGTFTKSFSGSGGYI GGKKEIVDYL RMQSHSTTYA TSMSPVVAAQ LIRSLKITMG YEGNIGGMERIQQLKENIKY FRRRLKEMGF IIYGNDFSPV IPVLLYMPAK VSAFSRFLLK KKISVVVVGFPATSLPEGRA RFSMSSAHTR EMLDTVLEVV DELGDLLNVK YFPLKKSGRA ILYNKEGFDNEASFEEMHSE PEA

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