蛋白C(PROC)重组蛋白

蛋白C(PROC)重组蛋白

Recombinant Protein C (PROC)

PRO-C; VKDPC; PC; Vitamin K Dependent Protein C; Inactivator Of Coagulation Factors Va And VIIIa; Autoprothrombin IIA; Blood coagulation factor XIV; Activation peptide

[ PROPERTIES ]

Residues: Lys197~Lys436

Tags: Two N-terminal Tags, His-tag and GST-tag

Accession: P33587

Host: E. coli

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative

Predicted isoelectric point: 6.1

Predicted Molecular Mass: 58.7kDa

Accurate Molecular Mass: 50&80kDa as determined by SDS-PAGE reducing

conditions.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Note: The possible reasons that the actual band size differs from the predicted are as follows:

1. Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to

give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in ddH2O. 

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

KRDT DLEDELEPDP RIVNGTLTKQ GDSPWQAILL DSKKKLACGG VLIHTSWVLTAAHCVEGTKK LTVRLGEYDL RRRDHWELDL DIKEILVHPN YTRSSSDNDI ALLRLAQPATLSKTIVPICL PNNGLAQELT QAGQETVVTG WGYQSDRIKD GRRNRTFILT FIRIPLVARNE CV E VM K NV V SE NM L C A GII GD T R D A C D G D SG GPM V V F F R GTW F LV G LV SWGEGCGHTNN YGIYTK

[ REFERENCES ]

1. Jalbert L.R., et al. (1998) Thromb. Haemost. 79:310-316. 

2. Murakawa M., et al. (1994) Br. J. Haematol. 86:590-600. 

3. Isermann B., et al. (2003) Nat. Med. 9:331-337. 

4. Weijer S., et al. (2005) Blood 106:2761-2768.

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