细胞色素P450家族成员24A1(CYP24A1)重组蛋白

细胞色素P450家族成员24A1(CYP24A1)重组蛋白

Recombinant Cytochrome P450 24A1 (CYP24A1)

CP24; CYP24; P450-CC24; Vitamin D 24-Hydroxylase; 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial ; Cytochrome P450 Family 24 Subfamily A Polypeptide 1

[ PROPERTIES ]

Residues: His260~Arg514 (Accession # Q64441),

with two N-terminal Tags, His-tag and T7-tag. Host: E. coli

Subcellular Location: Mitochondrion.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 8.9

Predicted Molecular Mass: 33.2kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- H KRLNTKVWQA HTLAWDTIFKSVKPCIDHRL ERYSQQPGAD FLCDIYQQDH LSKKELYAAV TELQLAAVET TANSLMWILYNLSRNPQVQQ RLLREIQSVL PDNQTPRAED VRNMPYLKAC LKESMRLTPS VPFTTRTLDKPTVLGEYTLP KGTVLTLNTQ VLGSSEDNFE DADKFRPERW LEKEKKINPF AHLPFGVGKRMCIGRRLAEL QLHLALCWII QKYNIVATDS EPVEMLHLGI LVPSRELPIA FCPR

[ REFERENCES ]

1. Itoh S., et al. (1995) Biochim. Biophys. Acta 1264:26-28. 

2. Akeno N., et al. (1997) Endocrinology 138:2233-2240. 

3. Tsujikawa H., et al. (2003) Mol. Endocrinol. 17:2393-2403.

 4. St-Arnaud R., et al. (2000) Endocrinology 141:2658-2666.

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