醛脱氢酶7家族成员A1(ALDH7A1)重组蛋白
Recombinant Aldehyde Dehydrogenase 7 Family, Member A1 (ALDH7A1)
ATQ1; PDE; EPD; Antiquitin; Alpha-Aminoadipic Semialdehyde Dehydrogenase; Delta1-Piperideine-6-Carboxylate Dehydrogenease; P6c Dehydrogenase
[ PROPERTIES ]
Residues: Met1~Gln539 (Accession # P49419),
with N-terminal His-Tag.
Host: E. coli
Subcellular Location: Mitochondrion. Cytoplasm,
cytosol.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 8.2
Predicted Molecular Mass: 59.7kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGS-MW RLP RALCV HAAKTSK LSG PWS RPAAFMS TLLI NQPQYAWLKELGLREE NEGVYNGSWG GRGEVITTYC PANNEPIARV RQASVADYEE TVKKAREAWKIWADIPAPKR GEIVRQIGDA LREKIQVLGS LVSLEMGKIL VEGVGEVQEY VDICDYAVGLSRMIGGPILP SERSGHALIE QWNPVGLVGI ITAFNFPVAV YGWNNAIAMI CGNVCLWKGAPTTSLISVAV TKIIAKVLED NKLPGAICSL TCGGADIGTA MAKDERVNLL SFTGSTQVGKQVGLMVQERF GRSLLELGGN NAIIAFEDAD LSLVVPSALF AAVGTAGQRC TTARRLFIHESIHDEVVNRL KKAYAQIRVG NPWDPNVLYG PLHTKQAVSM FLGAVEEAKK EGGTVVYGGK
VMDRPGNYVE PTIVTGLGHD ASIAHTETFA PILYVFKFKN EEEVFAWNNE VKQGLSSSIFTKDLGRIFRW LGPKGSDCGI VNVNIPTSGA EIGGAFGGEK HTGGGRESGS DAWKQYMRRSTCTINYSKDL PLAQGIKFQ
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