钙离子转运ATP酶A2(ATP2A2)重组蛋白

钙离子转运ATP酶A2(ATP2A2)重组蛋白

Recombinant ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2)

ATP2B; DAR; SERCA2; Calcium pump 2; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle

[ PROPERTIES ]

Source: Prokaryotic expression. Host: E. coli

Residues: Ile315~Met756

Tags: Two N-terminal Tags, His-tag and GST-tag

Tissue Specificity: Liver, Pancreas, Lung. Subcellular Location: Endoplasmic reticulum membrane; Multi-pass membrane

protein. Sarcoplasmic reticulum membrane. Purity: >92%

Traits: Freeze-dried powder

Buffer formulation: 100mM NaHCO3, 500mM NaCl, pH8.3, containing 1mM

EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200ug/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive

Labeling. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.4

Predicted Molecular Mass: 78.2kDa

Accurate Molecular Mass: 78kDa as determined by SDS-PAGE reducing conditions.

 [ USAGE ]

Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of0.1-1.0 mg/mL. Do not vortex.

 [ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8

oC for one month. Aliquot and store at -80

oC for 12 months. 

Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37oC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date under

appropriate storage condition.

[ SEQUENCE ]

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