基质金属蛋白酶10(MMP10)重组蛋白

基质金属蛋白酶10(MMP10)重组蛋白

Recombinant Matrix Metalloproteinase 10 (MMP10)

STMY2; SL2; Stromelysin 2; Transin-2

[ PROPERTIES ]

Residues: Phe99~Cys476 (Accession # P09238),

with N-terminal His-Tag.

Host: E. coli

Subcellular Location: Secreted, extracellular

space, extracellular matrix. Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 5.5

Predicted Molecular Mass: 44.5kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSGSEF-FS SFPGMPKWRK THLTYRIVNY TPDLPRDAVD SAIEKALKVWEEVTPLTFSR LYEGEADIMI SFAVKEHGDF YSFDGPGHSL AHAYPPGPGL YGDIHFDDDEKWTEDASGTN LFLVAAHELG HSLGLFHSAN TEALMYPLYN SFTELAQFRL SQDDVNGIQSLYGPPPASTE EPLVPTKSVP SGSEMPAKCD PALSFDAIST LRGEYLFFKD RYFWRRSHWNPEPEFHLISA FWPSLPSYLD AAYEVNSRDT VFIFKGNEFW AIRGNEVQAG YPRGIHTLGFPPTIRKIDAA VSDKEKKKTY FFAADKYWRF DENSQSMEQG FPRLIADDFP GVEPKVDAVLQAFGFFYFFS GSSQFEFDPN ARMVTHILKS NSWLHC

[ REFERENCES ]

1. Bertini I., et al. (2004) J. Mol. Biol. 336:707-716. 

2. Wertz K., et al. (2004) Free Radic. Biol. Med. 37:654-670. 

3. Schnabl B., et al. (2005) Biochem. Biophys. Res. Commun. 335:87-96.

 4. Aung P.P., et al. (2006) Oncogene 25:2546-2557.

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