卵巢肿瘤蛋白1(OTUB1)重组蛋白

卵巢肿瘤蛋白1(OTUB1)重组蛋白

Recombinant Otubain 1 (OTUB1)

HSPC263; OTB1; OTU1; OTU Domain,Ubiquitin Aldehyde Binding 1; Deubiquitinating enzyme OTUB1; Ubiquitin-specific-processing protease OTUB1

[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]

Protein Protein Protein Protein Names: Otubain 1

Gene Names: OTUB1

Size: 100µg

Source: Recombinant

Expression Expression Expression Expression Host: E.coli

Function: Function: Function: Function: Hydrolase that can remove conjugated ubiquitin from proteins in vitro and

may therefore play an important regulatory role at the level of protein turnover by

preventing degradation. Regulator of T-cell anergy, a phenomenon that occurs when

T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their

cognate antigen. Acts via its interaction with RNF128/GRAIL. Surprisingly, it regulates

RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128.

Deubiquitinates estrogen receptor alpha (ESR1). Mediates deubiquitination of

'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. Not able

to cleave di-ubiquitin. Also capable of removing NEDD8 from NEDD8 conjugates, but

with a nuch lower preference compared to 'Lys-48'-linked ubiquitin.

Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Cytoplasm

[ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]

Residues Residues Residues Residues: Met1~Lys271 (Accession # Q7TQI3), with a N-terminal His-tag.

Grade & Purity: Purity: Purity: Purity: >97%, 32.51 kDa as determined by SDS-PAGE reducing conditions.

Form & Buffer: Supplied as lyophilized form in PBS, pH 7.4.

Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method). Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 32.51 kDa

[ PREPARATION PREPARATION PREPARATION PREPARATION ]

Reconstitute in PBS.

[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]

Storage: Storage: Storage: Storage: Store at 4oC for short time storage (1-2 weeks). Aliquot and store at -20oC or -80oC for long term storage.

Avoid repeated freeze/thaw cycles.

Valid period: period: period: period: 12 months stored at -80oC.

[ BACKGROUND BACKGROUND BACKGROUND BACKGROUND]

The target protein is fused with a His-tag and its sequence is listed below. The first Met is an initiator amino acid. Moreover, Gly and Ser are added to improve the flexibility of N-terminus at both ends of the His-tag, which will

increase the chelating ability of the tag to Ni-Sepharose during purification.

MGHHHHHHSGS-MAAEEPQQQK QEPLGSDSEG VNCLAYDEAI MAQQDRIQQE IAVQNPLVSE RLELSVLYKEYAEDDNIYQQ KIKDLHKKYS YIRKTRPDGN CFYRAFGFSH LEALLDDSKE LQRFKAVSAK SKEDLVSQGFTEFTIEDFHN TFMDLIEQVE KQTSVADLLA SFNDQSTSDY LVVYLRLLTS GYLQRESKFF EHFIEGGRTV

KEFCQQEVEP MCKESDHIHI IALAQALSVS IQVEYMDRGE GGTTNPHVFP EGSEPKVYLL YRPGHYDILY K

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