丝裂原激活蛋白激酶激酶4(MAP2K4)重组蛋白

丝裂原激活蛋白激酶激酶4(MAP2K4)重组蛋白

Recombinant Mitogen Activated Protein Kinase Kinase 4 (MAP2K4)

JNKK; JNKK1; MAPKK4; MEK4; MKK4; PRKMK4; SEK1; SERK1; SKK1; c-Jun N-terminal kinase kinase 1; JNK-activating kinase 1; Stress-activated protein kinase kinase 1

[ PROPERTIES ]

Residues: Val137~Ser394

Tags: Two N-terminal Tags, His-tag and GST-tag

Accession: P45985

Host: E. coli

Subcellular Location: Cytoplasm. Nucleus.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 5.9

Predicted Molecular Mass: 61.3kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

 Store at 2-8

oC for one month.

 Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

VDEK EQKQLLMDLD VVMRSSDCPY IVQFYGALFR EGDCWICMEL MSTSFDKFYKYVYSVLDDVI PEEILGKITL ATVKALNHLK ENLKIIHRDI KPSNILLDRS GNIKLCDFGISGQLVDSIAK TRDAGCRPYM APERIDPSAS RQGYDVRSDV WSLGITLYEL ATGRFPYPKWNSVFDQLTQV VKGDPPQLSN SEEREFSPSF INFVNLCLTK DESKRPKYKELLKHPFILMYEERAVEVACY VCKILDQMPA TPSS

[ REFERENCES ]

1. Derijard B., et al. (1995) Science 267:682-685.

2. Lin A., et al. (1995) Science 268:286-290.

3. Su G.H., et al. (1998) Cancer Res. 58:2339-2342.

4. Tibbles L.A., et al. (1996) EMBO J. 15:7026-7035.

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