肾脏脑蛋白(KIBRA)重组蛋白

肾脏脑蛋白(KIBRA)重组蛋白

Recombinant Kidney And Brain Protein (KIBRA)

WWC1; WW Domain Containing Protein 1; WW,C2 And Coiled-Coil Domain Containing 1; HBeAg-binding protein 3

[ PROPERTIES ]

Source: Prokaryotic expression. Host: E. coli

Residues: Glu847~Met1094

Tags: N-terminal His-Tag

Tissue Specificity: Mammary Gland, Mammary Tumor, Salivary Gland, Liver. Subcellular Location: Cell membrane. Cell projection. Cytoplasm. Membrane. Nucleus. Purity: >98%

Traits: Freeze-dried powder

Buffer formulation: 100mM NaHCO3, 500mM NaCl, pH8.3, containing 1mM

EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 9.0

Predicted Molecular Mass: 32.4kDa

Accurate Molecular Mass: 40kDa as determined by SDS-PAGE reducing conditions. Phenomenon explanation:

The possible reasons that the actual band size differs from the predicted are as

follows:

1. Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to

give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. 

[ USAGE ]

Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8

oC for one month. Aliquot and store at -80

oC for 12 months.

 Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37oC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date under

appropriate storage condition. 

[ SEQUENCE ]

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