表面活性物质关联蛋白A2(SPA2)重组蛋白

表面活性物质关联蛋白A2(SPA2)重组蛋白

Recombinant Surfactant Associated Protein A2 (SPA2)

SFTPA2B; COLEC5; SP2A; SPA1 beta; SP-A2; SPAII; Collectin-5; Pulmonary Surfactant Protein A2; 35 kDa pulmonary surfactant-associated protein; Alveolar proteinosis protein

[ PROPERTIES ]

Residues: Asn21~Phe248

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: P08427

Host: E. coli

Subcellular Location: Secreted, extracellular

space, extracellular matrix.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 4.7

Predicted Molecular Mass: 27.9kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

NVTDVCAGSP GIPGAPGNHG LPGRDGRDGV KGDPGPPGPM GPPGGMPG LPGRDGLPGAPG APGERGDKGE PGERGLPGFP AYLDEELQTE LYEIKHQILQ TMGVLSLQGSMLSVGDKVFS TNGQSVNFDT IKEMCTRAGG NIAVPRTPEE NEAIASIAKK YNNYVYLGMIEDQTPGDFHY LDGASVNYTN WYPGEPRGQG KEKCVEMYTD GTWNDRGCLQ YRLAVCEF

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