CUB域EGF样信号肽3(SCUBE3)重组蛋白
Recombinant Signal Peptide, CUB Domain, EGF Like 3 (SCUBE3)
CEGF3; CUB Domain And EGF-Like Repeat Containing 3; Signal peptide, CUB and EGF-like domain-containing protein 3
[ PROPERTIES ]
Residues: Glu807~Lys993
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: Q8IX30
Host: E. coli
Subcellular Location: Secreted. Cell surface.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 7.9
Predicted Molecular Mass: 25.5kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
ELGE FTGYIESPNY PGNYPAGVEC IWNINPPPK R KILIVVPEIF LPSEDE CGDVLVMRKNSSPS SITTYETCQT YERPIAFTAR SRKLWINFKT SEANSARGFQ IPYVTYDEDYEQLVEDIVRD GRLYASENHQ EILKDKKLIK AFFEVLAHPQ NYFKYTEKHK EMLPKSFIKLLRSKVSSFLR PYK
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