Ⅴ型胶原α2(COL5a2)重组蛋白

Ⅴ型胶原α2(COL5a2)重组蛋白

Recombinant Collagen Type V Alpha 2 (COL5a2)

COL5-A2; Collagen Alpha-2(V)chain; AB Collagen

[ PROPERTIES ]

Residues: Cys82~Glu308 (Accession # P51861),

with two N-terminal Tags, His-tag and GST-tag. Host: E. coli

Subcellular Location: Secreted, extracellular

space, extracellular matrix.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 7.3

Predicted Molecular Mass: 54.1kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is less

than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its

sequence is listed below.

MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYIDGDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKVDFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFKKRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPRGSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-CADPVTPPGEC CPV CSQTP GGGNT NFGRG RKGQKGEPG L VPVVTGI RGR PGPAGPPGSQGPRGERGPKG RPGPRGPQGI DGEPGVPGQP GAPGPPGHPS HPGPDGLS RPFSAQMAGLDE KSGLGSQVGL MPGSVGPVGP RGPQGLQGQQ GGAGPTGPPGEPGDPGPMGP IGSRGPEGPP GKPGE DGEPG RNGNPGEVGF AGSPGARGFPGAPGLPGLKG HRGHKGLE

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