辅酶Q6同源物A(COQ6)重组蛋白

辅酶Q6同源物A(COQ6)重组蛋白

Recombinant Coenzyme Q6 Homolog, Monooxygenase (COQ6)

CGI-10; Coenzyme Q10 monooxygenase 6; Ubiquinone biosynthesis monooxygenase COQ6, mitochondrial

[ PROPERTIES ]

Residues: Met1~Lys468

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q9Y2Z9

Host: E. coli

Subcellular Location: Golgi apparatus. Cell

projection.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 6.8

Predicted Molecular Mass: 54.6kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

MAA R LVS R CG AV RAA P HSGP LVSW RRWS GA ST D TVY DV VV SGG G LV GAAMACALGYDIHF HDKKILLLEA GPKKVLEKLS ETYSNRVSSI SPGSATLLSS FGAWDHICNMRYRAFRRMQV WDACSEALIM FDKDNLDDMG YIVENDVIMH ALTKQLEAVS DRVTVLYRSKAIRYTWPCPF PMADSSPWVH ITLGDGSTFQ TKLLIGADGH NSGVRQAVGI QNVSWNYDQSAVVATLHLSE ATENNVAWQR FLPSGPIALL PLSDTLSSLV WSTSHEHAAE LVSMDEEKFVDAVNSAFWSD ADHTDFIDTA GAMLQYAVSL LKPTKVSARQ LPPSVARVDA KSRVLFPLGLGHAAEYVRPR VALIGDAAHR VHPLAGQGVN MGFGDISSLA HHLSTAAFNG KDLGSVSHLTGYETERQRHN TALLAATDLL KRLYSTSASP LVLLRTWGLQ ATNAVSPLKE QIMAFASK

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