角蛋白25(KRT25)重组蛋白

角蛋白25(KRT25)重组蛋白

Recombinant Keratin 25 (KRT25)

KRT25A; CK-25; K25; Cytokeratin-25; Keratin, type I cytoskeletal 25; Type I inner root sheath-specific keratin-K25irs1

[ PROPERTIES ]

Residues: Met1~Asn446

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q8VCW2

Host: E. coli

Subcellular Location: Cytoplasm.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 5.0

Predicted Molecular Mass: 52.6kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

MS L R L S S G S R RS YA R P S T GS LR G A S F GA G N A C GVA GI G S G FS C A F G GS S TGG N TGVA NS C AG F TV NE G G L LSG NE K V TM Q NL N D R L A SY L DNV QA L QEA NADLEQKIKGW YEKFGPGSCR GLDHDYSRYF PIIDDLKNQI ITSTTSNANA VLQIDNARLTADDFRLKYEN ELALHQSVEA DVNGLRRVLD EITLCRTDLE IQYETLSEEL TYLKKNHKEEMQA LQCAA GG NV NVEM NAA P GV D LTV L LN N MRA EYEA LAE QNRRDAEAW F

QEKSASLQQQ ITEDVGATTS ARNELTEMKR TLQTLEIELQ SLLATKHSLE CSLTETEGNYCTQLAQIQAQ ISALEEQLHQ VRTETEGQKL EYEQLLNVKA HLEKEIETYC LLIGGDEGACKSSSYKSKDY GSGNAGNQIK DPVKAIVVKK VLEEVDQRSK ILTTRLHSLE EKSQSN

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