酪氨酸激酶衔接蛋白非催化区1(NCK1)重组蛋白

酪氨酸激酶衔接蛋白非催化区1(NCK1)重组蛋白

Recombinant Cytoplasmic Protein NCK1 (NCK1)

NCKalpha; NCKa; NCK adaptor protein 1; Non Catalytic Region Of Tyrosine Kinase Adaptor Protein 1; SH2/SH3 adaptor protein NCK-alpha

[ PROPERTIES ]

Residues: Ala2~Ser377

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: P16333

Host: E. coli

Subcellular Location: Cytoplasm, Endoplasmic

reticulum, Nucleus.

Purity: >90%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 6.1

Predicted Molecular Mass: 46.4kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

AEEVVVVAK FDYVAQQEQE LDIKKNERLW LLDDSKSWWR VRNSMNKTGF VPSNYVERKNSARKASIVKN LKDTLGIGKV KRKPSVPDSA SPADDSFVDP GERLYDLNMP AYVKFNYMAERE DE LS LI KG TKVIVME K CS DGWW R GSY NG QV GW FPS NYV TEE GDS P LG DHVGSLSEKLA AVVNNLNTGQ VLHVVQALYP FSSSNDEELN FEKGDVMDVI EKPENDPEWWKCRKINGMVG LVPKNYVTVM QNNPLTSGLE PSPPQCDYIR PSLTGKFAGN PWYYGKVTRH

QAEMALNERG HEGDFLIRDS ESSPNDFSVS LKAQGKNKHF KVQLKETVYC IGQRKFSTMEELVEHYKKAP IFTSEQGEKL YLVKHLS

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