肌球蛋白重链11(MYH11)重组蛋白

肌球蛋白重链11(MYH11)重组蛋白

Recombinant Myosin Heavy Chain 11, Smooth Muscle (MYH11)

AAT4; FAA4; SMHC; SMMHC; Myosin heavy chain, smooth muscle isoform

[ PROPERTIES PROPERTIES PROPERTIES PROPERTIES ]

Residues: Residues: Residues: Residues: Lys211~Trp482 (Accession # Q63862),

with N-terminal His-Tag.

Host: E. coli

Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Melanosome. Cytoplasm,

myofibril.

Purity: Purity: Purity: Purity: >95%

Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 6.0

Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 32.6kDa

Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]

Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSG SEF-KVVSSVLQLG NIVFKKERNT DQASMPDNTA AQKVCHLVGINVTDFTRAIL TPRIKVGRDV VQKAQTKEQA DFAIEALAKA TYERLFRWIL SRVNKALDKTHRQGASFLGI LDIAGFEIFE VNSFEQLCIN YTNEKLQQLF NHTMFILEQE EYQREGIEWNFIDFGLDLQP CIELIERPNN PPGVLALLDE ECWFPKATDK SFVEKLCSEQ GNHPKFQKPKQLKDKTEFSI IHYAGKVDYN ASAWLTKNMD PLNDNVTSLL NASSDKFVAD LW

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！