信号淋巴细胞激活分子家族成员5(SLAMF5)重组蛋白

信号淋巴细胞激活分子家族成员5(SLAMF5)重组蛋白

Recombinant Signaling Lymphocytic Activation Molecule Family, Member 5 (SLAMF5)

CD84; LY9B; Cell surface antigen MAX.3; Hly9-beta; Leukocyte differentiation antigen CD84

[ PROPERTIES ]

Residues: Ile32~Leu222

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q18PI6

Host: E. coli

Subcellular Location: Cell membrane; Single-pass

type I membrane protein.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 5.8

Predicted Molecular Mass: 25.3kDa

Accurate Molecular Mass: 29kDa as determined by SDS-PAGE reducing conditions.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Note: The possible reasons that the actual band size differs from the predicted are as follows:

1. Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to

give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

ILGESVTFL LNIQEPKKID NIAWTSQSSV AFIKPGVNKA EVTITQGTYK GRIEIIDQKYDLVIRDLRME DAGTYKADIN EENEETITKI YYLHIYRRLK TPKITQSLIS SLNNTCNITLTCSVEKEEKD VTYSWSPFGE KSNVLQIVHS PMDQKLTYTC TAQNPVSNSS DSVTVQQPCTDTPSFHPRHA VL

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